+91 98677 24577
info@impactpvt.in
Plot No.101, College Phata, Panvel, Navi Mumbai

METANOR

Bacteria are a natural part of the water: purified drinking water contains about 20’000 to 300’000 bacterial cells per milliliter. Increasing water demand and deteriorating natural water quality are a constant concern, particularly for the water treatment industry. Selective laboratory measurements are intermittent and are no longer sufficient for current quality standards. Permanent water monitoring has become a necessity.

Measurement systems and sensors for continuous on-site monitoring have become increasingly important and already represent the state of technology for many water providers. These online measurement systems cover physical, optical, and chemical parameters such as temperature, flow, pressure, conductivity, turbidity (NTU), pH, monochloramine, fluorine compounds, and nitrates. Missing so far was a reliable on-site measurement that captures the bacterial activity of the water within short intervals and records the general and hygienic microbiological state.

ONLINE BACTERIA ANALYZER (OBA) -

Online Bacteria Analyzer closes a gap in online monitoring

The Online Bacteria Analyzer is the first autonomous measurement device that meets the complex requirements for use along the entire water production and distribution chain. All required preparation steps have been integrated into a single, process-suited device that provides measured data and results online. It is designed for continuous operation, 24 hours a day, 7 days a week. The device measures without human intervention for at least two weeks, then consumables like sheath fluid, pure water, and stain have to be replaced. OBA’s fail-safe system handles occurring interferences and compensates them.

FLOW CYTOMETRY

High resolution flow cytometric detection with OBA Smart Detect™

By staining water samples with DNA dyes, the flow cytometry technology can differentiate between organic cells and DNA-free, inorganic particles. SYBR green induces a green fluorescence on DNA or RNA. Propidium Iodide can permeate the damaged membrane of dead cells, but cannot permeate those of intact and living cells.

The stained cells and particles are then streamed through a glass capillary where they are illuminated by a focused laser beam. The scattered light of cells/particles and the emitted fluorescence of the dye are then collected through appropriate detectors.

OBA’s detection is based on this flow cytometry technology. The device distinguishes itself due to its optical design, the high sampling rate of the optical sensor with 2 MS/s or 4 MS/s, and the 24-bit dynamic range of its signal converters.

Due to the OBA SmartDetect™ detection algorithm it is no longer necessary to define a traditional signal threshold. OBA is immune to zero point offsets, caused by varying samples.

MEASUREMENT RESULTS -

Individual water samples differentiate by their microbiological structure. After the measurement of a sample, the results of two of the different scatter and fluorescence detectors can be combined with so-called dot-plots.

By defining regions inside the dot-plots (gating) Information about specific cell types can be retrieved like the total cell count (TCC), the amount of LNA (low nucleic acid cells), and HNA (high nucleic acid cells) and alive and dead cells.

Traditional methods at laboratories to determine the bacteria that form colonies of heterotrophic bacteria (HPC or heterotrophic plate count) take up to 48 or 72 hours until there are results. OBA reduces measurement time significantly: precise, real, and high-resolution results on the microbial state of the water are available within minutes.

MONITORING

OBA online monitoring is based on comparing the results of two or more consecutive measurements. Measuring the same sample source under constant conditions will result in minor deviations only. In case of a real event, such as infiltration by rainwater, contamination by manure, or a malfunction within the treatment process the results inside the scatter and fluorescence plots will change immediately.

By exceeding predefined thresholds a warning or alarm will be triggered: acoustic, optical, by email, by output signals to a logic controller (PLC), and by ethernet (Modbus/TCP). One can respond quickly to microbiological events.

Software

Powerful, comprehensive control and analysis software™

The Online Bacteria Analyzer has a powerful and comprehensive control and analysis software, focusing on ease of use without limiting possibilities for the user. Flexibility was and is considered an important Feature. By using predefined Templates one can start using the OBA in no time.

The software application offers a wide variety of usage; Ranging from a basic user to start a single measurement or measurement-sequences to the power user that parametrizes sample preparations and measurements or defines detailed processes and sequences.

Furthermore, the software application includes an extensive analysis toolset to evaluate data by given criteria after each measurement. The integrated database guarantees save data handling and data backup. By using the OBA Data Analyzer application data can be accessed remotely and then processed in a desktop environment

FACTS

Measurement results within 15 minutes
Closes a gap in online monitoring
Online operation on site where monitoring is required
Operates at least two weeks without intervention
Detection of non-culturable organisms possible
Automatic detection of alive and damaged (dead) cells
Early detection of malfunctions, defects or external influences
Maximum security; 24 hours, 7 days a week
Designed for the use in process and industrial environment
Two sample ports, one single-port for individual measurements

Location of Use

Drinking Water

Water suppliers
Quality control laboratories
Research
Food and beverage industry
Hospitals

Process Water: Ultrapure and High Purity Water

Pharmaceutical and chemical industry
Food industry
Cosmetic industry
Electroplating
Semiconductor industry

Biological Contamination of Drinking Water by Sabotage

Airports, water tanks of passenger aircrafts
Government buildings
Convention centres
Fairgrounds
Major events, state meetings
Potentially endangered objects

Specifications

Technical Specifications -

1	Measuring volume	50...200 µl
2	Measurement speed	Regular mode 1 µl/s or fast mode 2 µl/s
3	Cell and particle size	maximum of 5 µm
4	Measuring interval Evaluation	15 min to 360 min, depending on incubation time and process parameters through definition of areas (gates) in the data plots
5	Number of pumps	4
6	Pump type	High precision syringe pumps
7	Syringe volume	250, 500, 1’000 und 5’000 µl
8	Mixing ratio stain/sample	from 1:1…1:20
9	Incubation	20…45°C +/-0.5, resolution 0.1°C
10	Incubation time	min. 120 s, max. 1‘200 s, resolution 1 s
11	Mixer, incubator	chip technology
12	Dye (stain)	two containers inside the device
13	Dye (stain) temperature	from +10…+25°C
14	Sample port	2 sample port, 1 single-port for individual measurements
15	Light source	Laser, 488 nm, light power 30 mW
16	Optical detection	fluorescent colors (FL1 ... FL3) and a side scatter light channel
17	Detector type	Photomultiplier (PMT)
18	Data acquisition	4 Kanal, 24 Bit, 2 MS/s bzw. 4 MS/s
19	Maximum event rate	30’000 per second
20	Signal processing Algorithm	OBA SmartDetect™
21	Alarm types	Warning, alarm
22	Alarm definitions	when a predefined threshold is exceeded within a gate or within a combination of gates and over several measurements
23	Forwarding of alarms	via Modbus/TCP, PLC (SPS), e-mail
24	Control computer	integrated ATX computer, Intel Core™ i7 processor
25	Operating system	Linux
26	Operating of device	by external keyboard, mouse and monitor
27	Remote access	via Ethernet
28	Ethernet	standard RJ45
29	Control system	Modbus/TCP
30	PLC (SPS) Interface	analog outputs, 2 x 4...20 mA or 0...5 VDC digital in- and outputs
31	Ultrapure water	Canisters of 5 litres
31	Sheath fluid	Canisters of 5 litres
32	Cleaning solution	Canisters of 2.5 litres
33	Dye (stain)	two cartridges of 100 ml
34	Ambient temperature	5…35°C
35	Humidity	10 - 90% Rh, not condensing
36	Power	100...240 VAC, 50/60 Hz, 250 VA
37	Dimensions	width 460, height 590, depth 495 mm
38	Cabinet	sheet housing, powder coated
39	Weight	75 kg
		We reserve the right to change specifications at any time, without notice

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